The effect of L-arginine supplementation on VEGF and endostatin responses during exhaustive aerobic exercise in wrestlers

Document Type : Research Paper I Open Access I Released under (CC BY-NC 4.0) license

Authors

1 Department of Exercise Physiology, Faculty of Humanities and Social Sciences, University of Kurdistan, Sanandaj, Iran.

2 Department of Physical Education and Sport Sciences, Faculty of Humanities and Social Sciences, University of Kurdistan, Sanandaj, Iran.

Abstract

Aim: This study investigates the effect of L-arginine supplementation on VEGF and endostatin responses during a session of Bruce exhaustive protocol in wrestlers.

Method: Fifteen wrestlers, with a mean age of 21.13±2.47 years and a body mass index of 24.1±2.3 kg/m², were selected through voluntary and purposive sampling and randomly assigned to two groups: supplement (n=8) and placebo (n=7). In both the pre-test and post-test, subjects performed the Bruce exhaustive protocol until exhaustion, and blood samples were taken before and immediately after the protocol. Subjects in the supplement group received L-arginine supplementation (0.1 g/kg body weight) for 45 days, while the placebo group received maltodextrin. Both groups continued their specialized training during the supplementation period as in previous weeks. Serum VEGF and endostatin levels were measured using ELISA. Statistical analysis of the data was performed using paired and independent t-tests.

Results: Changes in VEGF, endostatin, and the VEGF to endostatin ratio in wrestlers after a period of arginine supplementation and following a session of the Bruce exhaustion test were not statistically significant (P > 0.05).

Conclusion: Although the findings of this study were not statistically significant, it appears that L-arginine supplementation, with a relative increase in VEGF and the VEGF to endostatin ratio, and a decrease in endostatin in the supplementation group, may contribute to the development of the angiogenesis process.

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Publisher: University of Kurdistan         Copyright © The Authors

This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

 

References

  • [1] Faraji H, Rahimi MR, Taeimouri S. The Effect of Salvia Officinalis Extract on P53 and Creatine Kinase Levels in Downhill Running: A Crossover Randomized, Double-Blind, and Placebo-Controlled Study. Research in Exercise Nutrition. 2022;1(1):29-3, Doi: https://doi.org/10.34785/J019.2022.392 .
  • [2] Hellsten Y, Nyberg M. Cardiovascular adaptations to exercise training. Comprehensive physiology. 2011;6(1):1-32, Doi: 10.1002/cphy.c140080.
  • [3] Mirzaei B, Curby DG, Rahmani-Nia F, Moghadasi M. Physiological profile of elite Iranian junior freestyle wrestlers. The Journal of Strength & Conditioning Research. 2009;23(8):2339-44, Doi: https://journals.lww.com/nsca-jscr/fulltext/2009/11000/Physiological_Profile_of_Elite_Iranian_Junior.21.aspx .
  • [4] Swanwick E, Matthews M. Energy systems: A new look at aerobic metabolism in stressful exercise. MOJ Sports Med. 2018;2(1):00039, Doi: http://dx.doi.org/10.15406/mojsm.2017.02.00039 .
  • [5] Rahimi MR, Mehrwand Z. The Impact of Resistance Training on IL-6, TNF-a, and CRP Levels in the Elderly: A Systematic Review and Meta-Analysis Study. International Journal of Sport Studies for Health. 2023;6(2), Doi: https://orcid.org/0000-0002-4302-1472 .
  • [6] Rahimi MR, Shoker-Nejad H. Effects of β-Hydroxy-β-Methylbutyrate Supplementation on IL-4, IL-10 and TGF-β1 during Resistance Exercise in Athletes. Research in Exercise Nutrition. 2022;1(1):40-21, doi: https://doi.org/10.34785/J019.2022.524 .
  • [7] Amaral SL, Papanek PE, Greene AS. Angiotensin II and VEGF are involved in angiogenesis induced by short-term exercise training. American Journal of Physiology-Heart and Circulatory Physiology. 2001;281(3):H1163-H9, Doi: https://doi.org/10.1152/ajpheart.2001.281.3.H1163 .
  • [8] Karamysheva A. Mechanisms of angiogenesis. Biochemistry (Moscow). 2008;73:751-62, doi: https://doi.org/10.1134/S0006297908070031 .
  • [9] Green DJ, Hopman MT, Padilla J, Laughlin MH, Thijssen DH. Vascular adaptation to exercise in humans: role of hemodynamic stimuli. Physiological reviews. 2017;97(2):495-528, Doi: https://doi.org/10.1152/physrev.00014.2016 .
  • [10] Sakellariou XM, Papafaklis MI, Domouzoglou EM, Katsouras CS, Michalis LK, Naka KK. Exercise-mediated adaptations in vascular function and structure: Beneficial effects in coronary artery disease. World Journal of Cardiology. 2021;13(9):399, Doi: http://dx.doi.org/10.4330/wjc.v13.i9.399 .
  • [11] Bloor CM. Angiogenesis during exercise and training. Angiogenesis. 2005;8(3):263-71, Doi: https://doi.org/10.1007/s10456-005-9013-x .
  • [12] Gorski T, De Bock K. Metabolic regulation of exercise-induced angiogenesis. Vascular Biology. 2019;1(1):H1-H8, Doi: https://doi.org/10.1530/VB-19-0008 .
  • [13] Shah I, Arif T, Ali I. The Effects of High Intensity Exercise to Exhaustion on the Concentrations of Endostatin and VEGF in Plasma: Effects of Exercise on Endostatin and VEGF concentrations. Pakistan BioMedical Journal. 2022:329-35, doi: https://doi.org/10.54393/pbmj.v5i6.590 .
  • [14] Shadmehri S, Ahmadi M. The Effect of Concurrent Endurance and Resistance Exercise on Plasma Levels of Vascular Endothelial Growth Factor and Endostatin in Inactive Women. Report of Health Care. 2016;2(1):38-45, Doi: https://jrhc.marvdasht.iau.ir/article_2794.html .
  • [15] Papapetropoulos A, García-Cardeña G, Madri JA, Sessa WC. Nitric oxide production contributes to the angiogenic properties of vascular endothelial growth factor in human endothelial cells. The Journal of clinical investigation. 1997;100(12):3131-9, Doi: https://doi.org/10.1172/JCI119868 .
  • [16] Suzuki J. Influence of amino acid supplementation on capillary growth in the heart and skeletal muscles. The Journal of Physical Fitness and Sports Medicine. 2013;2(2):237-41, Doi: https://doi.org/10.7600/jpfsm.2.237
  • [17] Bode‐Böger SM, Böger RH, Galland A, Tsikas D, Frölich JC. L‐arginine‐induced vasodilation in healthy humans: pharmacokinetic–pharmacodynamic relationship. British journal of clinical pharmacology. 1998;46(5):489-97, Doi: https://doi.org/10.1046/j.1365-2125.1998.00803.x .
  • [18] Carmeliet P. Angiogenesis in life, disease and medicine. Nature. 2005;438(7070):932-6, Doi: https://doi.org/10.1038/nature04478
  • [19] Aghjan S MN, Milani R,. Effects of L-arginine supplementation on response of VEGF and endostatin hind limb muscles of aged rats to acute exhaustive activity.: MSc Thesis Beheshti University.; 2012, Doi: https://doi.org/10.2170/physiolsci.rp000505
  • [20] Ranjbar K, Nourshahi M, Hedayati M, TAHERI CH. Effect of gender and physical activity on serum vascular endothelial growth factor at rest and response to submaximal exercise. Iranian Journal of Endocrinology and Metabolism. 2011;13(3):294-300, doi: http://ijem.sbmu.ac.ir/article-1-1022-en.html .
  • [21] Motahari Rad M, Attarzadeh Hosseini SR. Response of vascular endothelial growth factor and endostatin to a session activity before and after a period of L-arginine supplementation in active men. Journal of Arak University of Medical Sciences. 2017;20(2):78-88, Doi: http://jams.arakmu.ac.ir/article-1-4733-en.html
  • [22] Motahari Rad M, Attarzadeh Hosseini SR. The Effect of Selected Wrestling Training with L-Arginine Supplementation on Angiogenic and Angiostatic Serum Markers in Elite Wrestlers. Sport Physiology. 2016;8(31):153-70, Doi: https://doi.org/10.22089/spj.2016.818.
  • [23] Fiorito C, Balestrieri ML, Crimi E, Giovane A, Grimaldi V, Minucci PB, et al. Effect of L-arginine on circulating endothelial progenitor cells and VEGF after moderate physical training in mice. International Journal of Cardiology. 2008;126(3):421-3, Doi: https://doi.org/10.1016/j.ijcard.2007.12.004 .
  • [24] Gambardella J, Khondkar W, Morelli MB, Wang X, Santulli G, Trimarco V. Arginine and endothelial function. Biomedicines. 2020;8(8):277, Doi: https://doi.org/10.3390/biomedicines8080277 .

Research in Exercise Nutrition
Volume 3, Issue 1
April 2024
Pages 81-73

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History

  • Receive Date: 09 February 2025
  • Revise Date: 19 February 2025
  • Accept Date: 19 February 2025
  • First Publish Date: 19 February 2025

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