Effect of Melissa Officinalis L. extract supplementation on eccentric training induced muscle damage and perceived pain

Document Type : Original Article

Authors

1 Associate professor in exercise physiology, Department of exercise physiology, Shiraz branch, Islamic Azad University, Shiraz, Iran.

2 Master of Sciences student in exercise physiology, Department of exercise physiology, Zand institute of higher education, Shiraz, Iran.

3 Assistant professor in exercise physiology, Department of exercise physiology, Zand institute of higher education, Shiraz, Iran.

Abstract

Introduction: Lemongrass (Melissa officinalis) has an effect on the nervous system, causing relaxation and an important role in modulating the inflammatory condition. The aim of present study was to examine the effect of Melissa Officinalis L. extract supplementation on eccentric training induced muscle damage response and perceived pain.

Materials: In this experimental study, sixteen healthy middle-age men (20 – 35 years of old) were divided into placebo (Plc) or supplement (S) group randomly according their muscle strength. Subjects in the S group consume 1.5 mg/kg of Melissa Officinalis L. extract for 14 days while subjects in the Plc group consume same dosage of dextrose. Then, all the subjects performed eccentric biceps curls with 120% of their 1RM on two occasions. Blood sample were taken before and after the supplementation and immediately after the eccentric training. Data were analyzed using repeated measure ANOVA and Bonferroni post hoc test.

Results: The study results indicated that LDH increased in Plc group after the eccentric training (p=0.01). Blood CK had not significant change after the intervention in both groups and perceived pain was increased in both groups (p=0.01).

Conclusion: In summary, it seems that Melissa Officinalis L. extract supplementation can be considered as an effective way to reduce intense exercise induced-muscle damage, but more research is needed to draw definitive conclusions.

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[1] Irawan R, Sulistyarto S, Kumaat NA, Rimawati N. Correlation of delayed onset muscle soreness and inflammation post-exercise induced muscle. Open Acc Macedonian J Med Sci. 2022; 10(A):1688-1694.
[2] Diep S. DOMS. Ultrasound Med Biol. 2019;45:S46.
Hasson S, Mundorf R, Barnes W, Williams J, Fujii M. Effect of pulsed ultrasound versus placebo on musclesoreness perception and muscular performance. Scand J Rehabil Med. 1990; 22:199-205.
[3] Fleckenstein J, Neuberger EW, Bormuth P, Comes F, Schneider A, Banzer W, et al. Investigation of the sympathetic regulation in delayed onset muscle soreness: Results of an RCT. Front Physiol. 2021;12:1-13.
[4] Kaczmarek A, Kaczmarek M, Ciałowicz M, Clemente FM, Wolański P, Badicu G, et al. The role of satellite cells in skeletal muscle regeneration-the effect of exercise and age. Biology (Basel). 2021;10(10):1056.
[5] Shen L, Meng X, Zhang Z, Wang T. Physical exercise for muscle atrophy. Adv Exp Med Biol. 2018;1088:529-45.
[6] Hayashi K, Katanosaka K, Abe M, Yamanaka A, Nosaka K, Mizumura K, et al. Muscular mechanical hyperalgesia after lengthening contractions in rats depends on stretch velocity and range of motion. Eur J Pain. 2017;21(1):125-39.
[7] Pahwa R, Goyal A, Bansal P, Jialal I. Chronic inflammation. StatPearls Publishing LLC. 2020.
[8] Faraji H, Mmirahmad F, Mahammadi A. The effect of strawberry extract supplementation on some oxidative, inflammatory and cellular damage indicators after a session of exhausting resistance exercise in non-athlete women. Research in Exercise Nutrition. 2022;1(2):1-10.
[9] Bayat H, Khalounejad H, Babaei M, Arnavazi yamchi N, Azarbayjani MA. Interactive effect of a period of high-intensity interval training and cinnamon supplementation on the levels of some inflammatory and anti-inflammatory markers in overweight men. Research in Exercise Nutrition. 2022;1(2):35-42.
[10] Bayat H, Khalounejad H, Babaei M, Arnavazi yamchi N, Azarbayjani MA. Interactive effect of a period of high-intensity interval training and cinnamon supplementation on the levels of some inflammatory and anti-inflammatory markers in overweight men. Research in Exercise Nutrition. 2022;1(2):35-42.
[11] Akhondzadeh S, Noroozian M, Mohammadi M, Ohadinia S, Jamshidi A, Khani M. Melissa officinalis extract in the treatment of patients with mild to moderate Alzheimer's disease: a double blind, randomised, placebo-controlled trial. J Neurol Neurosurger Psychiatr. 2003; 74(7): 863-866.
[12] Eghbal H, Ghodrati L, Ahamdi sabegh M, Noorafkan H, Ghabaei T. Antibacterial activity of essential oil of lemon balm (Melissa Officinalis L). J Appl Chemist Enviroment. 2013; 4;14:47-52.
[13] Yengi Maleki Gh, Hemat-far A, Heidari N. Short-term effects of Melissa officinalis L. extract supplementation on inflammatory response and the perceived the pain of downhill running in young trained swimmers. Iran J Med Aromatic Plant. 2016; 32: 127-137.
[14] Jouris KB, McDaniel JL, Weiss EP. The effect of omega-3 fatty acid supplementation on the inflammatory response to eccentric strength exercise. J Sport Sci Med. 2011; 10; 432-438.
[15] Draginic N, Andjic M, Jeremic J, Zivkovic V, Kocovic A, Tomovic M, et al. Anti-inflammatory and antioxidant effects of Melissa officinalis extracts: A comparative study. Iran J Pharm Res. 2022; 21(1): e126561.
[16] Shakeri A, Sahebkar A, Javadi B. Melissa officinalis L. A review of its traditional uses, phytochemistry and pharmacology. J Ethnopharmacol. 2016;188:204–28.
[17] Ibragić S, Salihović M, Tahirović I, Toromanović J. Quantification of some phenolic acids in the leaves of Melissa officinalis L. from Turkey and Bosnia. Bull Chem Tech Bosnia Herzegovina. 2014;42:47–50. [Google Scholar]
[18] Arceusz A, Wesolowski M, Ulewicz-Magulska B. Flavonoids and Phenolic Acids in Methanolic Extracts, Infusions and Tinctures from Commercial Samples of Lemon Balm. Natural Prod Commun. 2015;10(6):977–81.
[19] Mimica-Dukic N, Bozin B, Sokovic M, Simin N. Antimicrobial and antioxidant activities of Melissa officinalis L. (Lamiaceae) essential oil. J Agric Food Chem. 2004;52(9):2485–9.
[20] Sedighi M, Faghihi M, Rafieian-Kopaei M, Rasoulian B, Nazari A. Cardioprotective effect of ethanolic leaf extract of melissa officinalis L against regional ischemia-induced arrhythmia and heart injury after five days of reperfusion in rats. Iran J Pharm Res. 2019;18(3):1530–42.
[21] Hamza AA, Ahmed MM, Elwey HM, Amin A. Melissa officinalis Protects against Doxorubicin-Induced Cardiotoxicity in Rats and Potentiates Its Anticancer Activity on MCF-7 Cells. PLoS One. 2016;11(11):e0167049.
[22] Drozd JANINA, Anuszewska E. The effect of the Melissa officinalis extract on immune response in mice. Acta Pol Pharm. 2003;60(6):467–70.
[23] Bounihi A, Hajjaj G, Alnamer R, Cherrah Y, Zellou A. In Vivo Potential Anti-Inflammatory Activity of Melissa officinalis L. Essential Oil. Adv Pharmacol Sci. 2013;2013:101759.
[24] Saini AK, Goyal R, Gauttam VK, Kalia AN. Evaluation of anti-inflammatory potential of Ricinus communis Linn leaves extracts and its flavonoids content in Wistar rats. J Chem Pharm Res. 2010;2(5):690–5.
[25] Haddad M, Cherchi F, Alsalem M, Al-saraireh YM, Madaeen S. Adenosine receptors as potential therapeutic analgesic targets. Int J Mol Sci. 2023; 24(17): 13160.
[26] Uritu CM, Mihai CT, Stanciu GD, Dodi G, Alexa-Stratulat T, Luca A, et al. Medicinal plants of the family lamiaceae in pain therapy: A review. Pain Res Manag. 2018; 2018: 7801543.
[27] Ricciotti E, FitzGerald GA. Prostaglandins and inflammation. Arterioscler Thromb Vasc Biol. 2011; 31(5): 986–1000.
[28] Mirabi P, Namdari M, Alamolhoda SH, Mojab F. The effect of Melissa officinalis extract on the severity of primary dysmenorrhea. Iran J Pharm Res. 2017; 16(Suppl): 171–177.
Volume 1, Issue 3
November 2022
Pages 53-60
  • Receive Date: 22 November 2023
  • Revise Date: 10 December 2023
  • Accept Date: 10 December 2023
  • First Publish Date: 10 December 2023