تأثیر مصرف عصاره مریم‌گلی بر سطوح سرمی کاسپاز 3 به دنبال یک جلسه دویدن در سراشیبی

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I

نویسندگان

1 گروه تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی، واحد مریوان، مریوان، ایران.

2 گروه تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی، واحد مریوان، مریوان، ایران

چکیده

هدف: هدف از تحقیق حاضر بررسی تأثیر مصرف عصاره مریم‌گلی بر سطوح سرمی کاسپاز 3 به دنبال یک جلسه دویدن در سراشیبی بود.

روش شناسی: 12 مرد سالم (سن: 75/2±38/24، وزن: 48/5±57/75، BMI: 70/1±69/24) به‌صورت تصادفی در دو گروه مکمل مریم‌گلی و دارونما به‌صورت متقاطع قرار گرفتند. آزمودنی‌ها به مدت دو هفته کپسول‌های 100 میلی‌گرمی عصاره مریم‌گلی (روزانه دو عدد) یا دارونما را مصرف کردند. پس از 2 هفته ، در پانزدهمین روز، یک جلسه فعالیت ورزشی نوار گردان با شیب منفی را انجام دادند. پروتکل ورزشی شامل یک جلسه فعالیت ورزشی با شیب منفی 12 درصد با شدت 70 درصد ضربان قلب بیشینه تا خستگی ارادی (میانگین خستگی ارادی آزمودنی‌ها 30 دقیقه بود) بود. خون‌گیری در دو مرحله قبل و بلافاصله بعد از جلسه ورزش انجام گرفت

یافته ها: بین گروه مکمل و گروه دارونما در سطوح سرمی کاسپاز 3 تفاوت معنی‌داری در پیش آزمون مشاهده نشد (134/0=p). نتایج آزمون تعقیبی بونفرونی نشان داد که در گروه مکمل تغییرات سطوح کاسپاز در پس‌آزمون نسبت به پیش‌آزمون از لحاظ آماری معنی‌دار نبود (650/0=p). در گروه دارونما سطوح کاسپاز 3 در پس‌آزمون نسبت به پیش‌آزمون به‌صورت معنی‌داری بالاتر بود (01/0=p).

نتیجه گیری: دویدن در سراشیبی به‌عنوان انقباضات اکسنتریک ممکن است از طریق افزایش سطوح کاسپاز 3 به آپوپتوز سلولی منجر شود، اما مصرف کوتاه‌مدت مکمل مریم‌گلی به‌احتمال‌زیاد منجر به مهار مرگ سلولی ناشی از ورزش می‌شود.

تازه های تحقیق

 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of Salvia Consumption on Serum Levels of Caspase-3 Following One Session Downhill Running

نویسندگان [English]

  • Hassan Faraji 1
  • Tarifa Babaie 2
1 Department of Physical Education and Sport Sciences, Islamic Azad University, Marivan Branch, Marivan, Iran.
2 department of physical education and sport sciences, Marivan branch, Islamic Azad University, Marivan, Iran
چکیده [English]

Aim: The purpose of this study was to investigate the effect of Salvia officinalis extract on the serum levels of caspase 3 after a downhill running session.

Method: 12 healthy men (age: 24.38±2.75, weight: 75.57±5.48, BMI: 24.69±1.70) were randomly assigned to two groups of sage supplement and placebo in a crossover manner. Subjects consumed 100 mg capsules of sage extract (two per day) or placebo for two weeks. After 2 weeks, they performed one session of exercise on a treadmill with a negative slope on one day. The exercise protocol included one session of exercise with a negative slope of 12% at an intensity of 70% of maximum heart rate until voluntary fatigue (the average voluntary fatigue of the subjects was 30 minutes). Blood sampling was performed in two stages before and immediately after the exercise session.

Results: There was no significant difference in serum caspase 3 levels between the supplement group and the placebo group in the pre-test (p=0.134). The results of the Bonferroni post-hoc test showed that in the supplement group, the changes in caspase levels in the post-test compared to the pre-test were not statistically significant (p=0.650). In the placebo group, the levels of caspase 3 in the post-test compared to the pre-test were significantly higher (p=0.01).

Conclusion: The running downhill as eccentric contractions may lead to cell apoptosis through increasing caspase 3 levels, but short-term consumption of Salvia officinalis supplement is likely to inhibit exercise-induced cell death.

کلیدواژه‌ها [English]

  • Apoptosis
  • Downhill
  • Salivia
  • Caspase 3

اصل مقاله

 

Publisher: University of Kurdistan         Copyright © The Authors

This is an open access article distributed under the following Creative Commons license: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

 

مراجع

  • [1] Kano Y, Sonobe T, Inagaki T, Sudo M, Poole DC. Mechanisms of exercise-induced muscle damage and fatigue: Intracellular calcium accumulation. The Journal of Physical Fitness and Sports Medicine. 2012;1(3):505-12, Doi: https://doi.org/10.7600/jpfsm.1.505 .
  • [2] Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell. 1997;91(4):479-89, Doi: https://doi.org/10.1016/S0092-8674(00)80434-1 .
  • [3] Rasheva VI, Domingos PM. Cellular responses to endoplasmic reticulum stress and apoptosis. Apoptosis. 2009;14(8):996-1007, Doi: https://doi.org/10.1007/s10495-009-0341-y .
  • [4] Rodríguez-Berriguete G, Galvis L, Fraile B, de Bethencourt FR, Martínez-Onsurbe P, Olmedilla G, et al. Immunoreactivity to caspase-3, caspase-7, caspase-8, and caspase-9 forms is frequently lost in human prostate tumors. Human pathology. 2012;43(2):229-37, Doi: https://doi.org/10.1016/j.humpath.2011.04.024 .
  • [5] Harada H, Hiraoka M, Kizaka-Kondoh S. Antitumor effect of TAT-oxygen-dependent degradation-caspase-3 fusion protein specifically stabilized and activated in hypoxic tumor cells. Cancer research. 2002;62(7):2013-8, Doi: https://aacrjournals.org/cancerres/article/62/7/2013/509786/Antitumor-Effect-of-TAT-Oxygen-dependent .
  • [6] Rahimi R, Khbiri P, Faraji H. Effects of caffeine ingestion on resistance exercise-induced apoptosis in athletes: A randomized, double-blind, placebo-controlled, crossover study. Progress in Nutrition. 2018;20(4):563-9, Doi: https://doi.org/10.23751/pn.v20i4.6442 .
  • [7] Powers SK, Jackson MJ. Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. Physiological reviews. 2008;88(4):1243-76, Doi: https://doi.org/10.1152/physrev.00031.2007 .
  • [8] Sudo M, Kano Y. Myofiber apoptosis occurs in the inflammation and regeneration phase following eccentric contractions in rats. The Journal of Physiological Sciences. 2009;59(6):405, Doi: https://doi.org/10.1007/s12576-009-0049-3 .
  • [9] Sun Y, Cui D, Zhang Z, Zhang T, Shi J, Jin H, et al. Attenuated oxidative stress following acute exhaustive swimming exercise was accompanied with modified gene expression profiles of apoptosis in the skeletal muscle of mice. Oxidative medicine and cellular longevity. 2016;2016, Doi: https://doi.org/10.1155/2016/8381242 .
  • [10] Quadrilatero J, Alway SE, Dupont-Versteegden EE. Skeletal muscle apoptotic response to physical activity: potential mechanisms for protection. Applied physiology, nutrition, and metabolism. 2011;36(5):608-17, Doi: https://doi.org/10.1139/h11-064 .
  • [11] Faraji H, Rahimi R, Sheikholeslami Vatani D, Jafari A. Apoptosis response to different rest periods after resistance exercise in athletes. Medicina Dello Sport. 2016;69(2):173-83, Doi: https://www.minervamedica.it/en/journals/medicina-dello-sport/article.php?cod=R26Y2016N02A0173 .
  • [12] Boroujerdi S, Rahimi R. The apoptotic response to resistance exercise with different intensities in athletes. Med Sport. 2011;64(1):31-44, Doi: https://www.minervamedica.it/en/journals/medicina-dello-sport/article.php?cod=R26Y2011N01A0031 .
  • [13] Sharafi H, Rahimi R. The effect of resistance exercise on p53, caspase-9, and caspase-3 in trained and untrained men. The Journal of Strength & Conditioning Research. 2012;26(4):1142-8, Doi: 10.1519/JSC.0b013e31822e58e5 .
  • [14] Sheikholeslami-Vatani D, Faraji H. Influence of Creatine Supplementation on Apoptosis Markers After Downhill Running in Middle-Aged Men: A Crossover Randomized, Double-Blind, and Placebo-Controlled Study. American journal of physical medicine & rehabilitation. 2018;97(11):825-31, Doi: 10.1097/PHM.0000000000000977 .
  • [15] Park K-S, Lee M-G. Effects of unaccustomed downhill running on muscle damage, oxidative stress, and leukocyte apoptosis. Journal of exercise nutrition & biochemistry. 2015;19(2):55, Doi: https://doi.org/10.5717/jenb.2015.15050702 .
  • [16] Park K-S, Sedlock DA, Navalta JW, Lee M-G, Kim S-H. Leukocyte apoptosis and pro-/anti-apoptotic proteins following downhill running. European journal of applied physiology. 2011;111(9):2349-57, Doi: https://doi.org/10.1007/s00421-011-1907-2 .
  • [17] Park K-S, Sedlock DA, Navalta JW. Exercise-induced muscle damage and apoptotic protein expression in immune cells. Federation of American Societies for Experimental Biology; 2007, Doi: https://doi.org/10.1096/fasebj.21.6.A1345 .
  • [18] Jantová S, Hudec R, Sekretár S, Kučerák J, Melušová M. Salvia officinalis L. extract and its new food antioxidant formulations induce apoptosis through mitochondrial/caspase pathway in leukemia L1210 cells. Interdisciplinary toxicology. 2014;7(3):146-53, Doi: 10.2478/intox-2014-0020 .
  • [19] Willcox JK, Ash SL, Catignani GL. Antioxidants and prevention of chronic disease. Critical reviews in food science and nutrition. 2004;44(4):275-95, Doi: https://doi.org/10.1080/10408690490468489 .
  • [20] Imanshahidi M, Hosseinzadeh H. The pharmacological effects of Salvia species on the central nervous system. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 2006;20(6):427-37, Doi: https://doi.org/10.1002/ptr.1898 .
  • [21]

45
  • Kuźma Ł, Skrzypek Z, Wysokińska H. Diterpenoids and triterpenoids in hairy roots of Salvia sclarea. Plant cell, tissue and organ culture. 2006;84(2):171-9, Doi: https://doi.org/10.1007/s11240-005-9018-6 .
  • [22] Lu Y, Foo LY. Polyphenolics of Salvia—a review. Phytochemistry. 2002;59(2):117-40, Doi: https://doi.org/10.1016/S0031-9422(01)00415-0 .
  • [23] Tayarani-Najaran Z, Mousavi SH, Tajfard F, Asili J, Soltani S, Hatamipour M, et al. Cytotoxic and apoptogenic properties of three isolated diterpenoids from Salvia chorassanica through bioassay-guided fractionation. Food and chemical toxicology. 2013;57:346-51, Doi: https://doi.org/10.1016/j.fct.2013.03.037 .
  • [24] Wu Y-B, Ni Z-Y, Shi Q-W, Dong M, Kiyota H, Gu Y-C, et al. Constituents from Salvia species and their biological activities. Chemical reviews. 2012;112(11):5967-6026, Doi: https://pubs.acs.org/doi/full/10.1021/cr200058f .
  • [25] Koçtürk S, Kayatekin B, Resmi H, Açıkgöz O, Kaynak C, Özer E. The apoptotic response to strenuous exercise of the gastrocnemius and solues muscle fibers in rats. European journal of applied physiology. 2008;102(5):515-24, Doi: https://doi.org/10.1007/s00421-007-0612-7 .
  • [26] Elmore S. Apoptosis: a review of programmed cell death. Toxicologic pathology. 2007;35(4):495-516, Doi: https://journals.sagepub.com/doi/full/10.1080/01926230701320337 .
  • [27] Yang Y, Jemiolo B, Trappe S. Proteolytic mRNA expression in response to acute resistance exercise in human single skeletal muscle fibers. Journal of applied physiology. 2006;101(5):1442-50, Doi: https://doi.org/10.1152/japplphysiol.00438.2006 .
  • [28] Rong Y, Distelhorst CW. Bcl-2 protein family members: versatile regulators of calcium signaling in cell survival and apoptosis. Annu Rev Physiol. 2008;70:73-91, Doi: https://doi.org/10.1146/annurev.physiol.70.021507.105852 .
  • [29] Phaneuf S, Leeuwenburgh C. Apoptosis and exercise. Medicine and science in sports and exercise. 2001;33(3):393-6, Doi: https://doi.org/10.1097/00005768-200103000-00010 .
  • [30] Warren GL, Ingalls CP, Lowe DA, Armstrong R. What mechanisms contribute to the strength loss that occurs during and in the recovery from skeletal muscle injury? Journal of Orthopaedic & Sports Physical Therapy. 2002;32(2):58-64, Doi: https://www.jospt.org/doi/10.2519/jospt.2002.32.2.58 .
  • [31] Gissel H. The role of Ca2+ in muscle cell damage. Annals of the New York Academy of Sciences. 2006;1066(1):166-80, Doi: https://doi.org/10.1196/annals.1363.013 .
  • [32] Nakagawa T, Yuan J. Cross-talk between two cysteine protease families: activation of caspase-12 by calpain in apoptosis. The Journal of cell biology. 2000;150(4):887-94, Doi: https://doi.org/10.1083/jcb.150.4.887 .
  • [33] Townsend JR, Stout JR, Jajtner AR, Church DD, Beyer KS, Riffe JJ, et al. Polyphenol supplementation alters intramuscular apoptotic signaling following acute resistance exercise. Physiological reports. 2018;6(2), Doi: https://doi.org/10.14814/phy2.13552 .
  • [34] Ghorbani A, Esmaeilizadeh M. Pharmacological properties of Salvia officinalis and its components. Journal of traditional and complementary medicine. 2017;7(4):433-40, Doi: https://doi.org/10.1016/j.jtcme.2016.12.014 .
  • [35] Kontogianni VG, Tomic G, Nikolic I, Nerantzaki AA, Sayyad N, Stosic-Grujicic S, et al. Phytochemical profile of Rosmarinus officinalis and Salvia officinalis extracts and correlation to their antioxidant and anti-proliferative activity. Food Chemistry. 2013;136(1):120-9, Doi: https://doi.org/10.1016/j.foodchem.2012.07.091 .
  • [36] El Hadri A, del Rio MG, Sanz J, Coloma AG, Idaomar M, Ozonas BR, et al. Cytotoxic activity of α-humulene and transcaryophyllene from Salvia officinalis in animal and human tumor cells. An R Acad Nac Farm. 2010;76(3):343-56, Doi: https://core.ac.uk/download/pdf/230311668.pdf .
  • [37] Lima CF, Carvalho F, Fernandes E, Bastos MdL, Santos-Gomes P, Fernandes-Ferreira M, et al. Evaluation of toxic/protective effects of the essential oil of Salvia officinalis on freshly isolated rat hepatocytes. Toxicology in vitro. 2004;18(4):457-65, Doi: https://doi.org/10.1016/j.tiv.2004.01.001 .
  • [38] Horváthová E, Srančíková A, Regendová-Sedláčková E, Melušová M, Meluš V, Netriová J, et al. Enriching the drinking water of rats with extracts of Salvia officinalis and Thymus vulgaris increases their resistance to oxidative stress. Mutagenesis. 2015;31(1):51-9, Doi: https://doi.org/10.1093/mutage/gev056 .
  • [39] Kozics K, Klusová V, Srančíková A, Mučaji P, Slameňová D, Hunáková Ľ, et al. Effects of Salvia officinalis and Thymus vulgaris on oxidant-induced DNA damage and antioxidant status in HepG2 cells. Food chemistry. 2013;141(3):2198-206, Doi: https://doi.org/10.1016/j.foodchem.2013.04.089 .
  • [40] Cuvelier ME, Richard H, Berset C. Antioxidative activity and phenolic composition of pilot‐plant and commercial extracts of sage and rosemary. Journal of the American Oil Chemists' Society. 1996;73(5):645-52, Doi: https://doi.org/10.1007/BF02518121 .
  • [41] Azevedo MI, Pereira AF, Nogueira RB, Rolim FE, Brito GA, Wong DVT, et al. The antioxidant effects of the flavonoids rutin and quercetin inhibit oxaliplatin-induced chronic painful peripheral neuropathy. Molecular pain. 2013;9(1):53. Doi: https://doi.org/10.1186/1744-8069-9-53 .
  • [42] Mansourabadi AH, Sadeghi HM, Razavi N, Rezvani E. Anti-inflammatory and analgesic properties of Salvigenin, Salvia officinalis flavonoid extracted. Advanced Herbal Medicine. 2015;1(3):31-41, Doi: https://fnp.skums.ac.ir/Article/fnp-23 .
  • [43] Osakabe N, Yasuda A, Natsume M, Yoshikawa T. Rosmarinic acid inhibits epidermal inflammatory responses: anticarcinogenic effect of Perilla frutescens extract in the murine two-stage skin model. Carcinogenesis. 2004;25(4):549-57, Doi: https://doi.org/10.1093/carcin/bgh034
  • [44] Baricevic D, Sosa S, Della Loggia R, Tubaro A, Simonovska B, Krasna A, et al. Topical anti-inflammatory activity of Salvia officinalis L. leaves: the relevance of ursolic acid. Journal of ethnopharmacology. 2001;75(2-3):125-32, Doi: https://doi.org/10.1016/S0378-8741(00)00396-2

پژوهش در تغذیه ورزشی
دوره 3، شماره 1
فروردین 1403
صفحه 46-37

فایل ها

سابقه مقاله

  • تاریخ دریافت: 18 دی 1403
  • تاریخ بازنگری: 06 بهمن 1403
  • تاریخ پذیرش: 16 بهمن 1403
  • تاریخ اولین انتشار: 16 بهمن 1403

هم رسانی

ارجاع به این مقاله

آمار